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TITAN GEL Agarose System
1. Protein
2. ImmunoFixation
3. Immunoelectrophoresis
4. High Resolution Proteins
5. Alkaline Phosphatase Isoenzymes
Product Detail

1. TITAN GEL Protein Electrophoresis  

QuickGel Cholesterol Profile Gel

The key roles which serum proteins play in bodily functions and the relative ease of separating them by electrophoresis, make their determination a valuable diagnostic tool as well as a way to monitor clinical progress. Total serum protein levels and serum protein electrophoresis should be included in every laboratory work-up on a patient. There is no other single procedure, or even group of procedures, that is capable of detecting so broad a spectrum of disease states or is as indicative of the degree of health as serum protein electrophoresis.

TITAN GEL Serum Protein Kit

  • Titan Gel Serum Protein Kit
Cat. No. Item Description
3041 TITAN GEL Serum Protein Kit 10 x 10 samples/gel


Kits include: 10 Gels, Serum Protein Buffer, Amido Black Stain, Templates, Blotters.

Five-band protein separation is quick and easy with the TlTAN GEL System. Ten samples may be run on each gel. Fixing and staining of proteins is accomplished in one step. The complete procedure requires less than 40 minutes. Kit contains all necessary gels and reagents.


Serum Protein Electrophoresis Control

  • Serum Protein Electrophoresis Control
Cat. No. Item Description
5136 SPE Control 1 x 2 mL


The control is for qualitative and/or quantitative use in agarose serum protein electrophoresis. It is a stabilized liquid prepared from human serum and assayed for total protein, albumin, alpha1, alpha2, beta and gamma globulins.

For SPIFE Serum Protein assays, order Cat. No. 3424 or 3425.


2. TITAN GEL Immunofixation


QuickGel Cholesterol Profile Gel


Immunofixation electrophoresis (IFE) is characterized by its enhanced sensitivity, ease of interpretation, quick test results, and excellent resolution. IFE is a two stage procedure using agarose protein electrophoresis first, then immunoprecipitation. The specimen may be serum, urine or CSF. There are many applications for IFE but the primary use is characterization of paraproteins. Distinct protein separation, antigen/antibody specificity, and direct comparison of IFE bands with the corresponding protein pattern from the same system make interpretation easy and provide definitive results. Pre-cut wells on each antisera lane allow use of Titan Gel ImmunoFix Controls to provide verification of antisera reactivity on each patient gel.


TITAN GEL ImmunoFix 6-Channel Gels and Antisera

  • Titan Gel ImmunoFix kit
Cat. No. Item Description
3046 TITAN GEL ImmunoFix Kit 10 x 6 channels/gel


Kits include: 10 Gels, Acid Blue Stain, IFE Buffer, Sample Templates, Antisera Templates, Protein Fixative, Blotters, and 1 x 0.75 mL vial each of Antisera to IgG, IgA, IgM, Kappa and Lambda.

Each TITAN GEL ImmunoFix Kit contains reagents and supplies for performing 10 patient profiles on a standard 6-channel gel. The procedure requires just over an hour to complete. Wash and press steps require only 10 minutes. Antisera are color coded and packaged in applicator vials for easier application and quality assurance. Each gel contains wells for application of TITAN GEL IFE Controls.


TITAN GEL IFE Antisera Kit

  • Titan Gel IFE Antisera Kit
Cat. No. Item
3068 TITAN GEL IFE Antisera Kit

Kits include: Protein Fixative and 1 x 0.75 mL vial each of Antisera to IgG, IgA, IgM, Kappa and Lambda.

This kit provides the routine monospecific antisera needed for TITAN GEL ImmunoFix Procedures. The high affinity and titer are excellent for detecting and identifying paraproteins. The Protein Fixative provides a complete protein pattern for comprehensive work-up. Color-coded antisera are packaged in applicator vials.


TITAN GEL IFE IgD and IgE Antisera

Cat. No. Item
9409 TITAN GEL IFE Antiserum to IgD, 1 x 1 mL
9410 TITAN GEL IFE Antiserum to IgE, 1 x 1 mL

When performing IFE, if a reaction occurs with kappa or lambda but no reaction occurs with the IgG, IgA, or IgM heavy chains, the patient sample should be retested with IgD and IgE antisera. A reaction indicates an IgD or IgE monoclonal gammopathy. Samples with a light chain reaction and no reaction with IgD or IgE are appropriately reported as Free Light Chain Disease. Packaged in convenient applicator vials.


3. TITAN GEL Immunoelectrophoresis


QuickGel Cholesterol Profile Gel


IEP is a reliable and accurate method for routine serum and urine protein evaluations, detecting both structural abnormalities and concentration changes. It has been widely used in identification of monoclonal gammopathies. Immunoelectrophoresis (IEP) combines two techniques, electrophoresis and immunodiffusion. In this two-part procedure, proteins are first separated by electrophoresis. Then antisera, complementary to the proteins under study, are applied to the gel and allowed to diffuse. When a favorable antigen to antibody ratio exists, a precipitin arc forms on the gel.

Both thick and thin gels are available for performing immunoelectrophoresis. The thick agarose, on a rigid plastic backing for ease of handling, requires a larger volume of antisera and patient samples, and forms a dense precipitin arc easily interpreted and photographed without staining the gel. The thin agarose gel, on a flexible plastic backing, provides economical use of antisera and lends itself to staining and drying of the gel for a permanent record.


TITAN GEL Immunoelectrophoresis

  • Quickgel Chamber
Cat. No. Item
3047 TITAN GEL IEPlate Kit, 10 IEPlates plus blotters


TITAN GEL IEPlates provide fast, economical immunoelectrophoresis. The thin gel uses a reduced sample volume of 2 mL and antisera volume of 25 mL which means tremendous cost savings. Each gel has eight wells and seven troughs to accommodate a wide variety of antisera profiles. After an 18-hour incubation, non-precipitated proteins are washed from the gel. The gel can then be stained, destained and dried within one hour. The printed plastic backing makes sample and control identification easy and includes a preprinted scale for measuring migration distance.

Cat. No. Item
9231 Antisera to Human IgA, Heavy Chain Specific, 1 x 2 mL
9232 Antisera to Human IgG, Heavy Chain Specific, 1 x 2 mL
9233 Antisera to Human Whole Serum, 1 x 2 mL
9234 Antisera to Human IgM, Heavy Chain Specific, 1 x 2 mL
9236 Antisera to Human (IgG, A, M) Trivalent, Heavy Chain Specific, 1 x 2 mL
9249 Antisera to Human IgD, Heavy Chain Specific, 1 x 2 mL
9250 Antisera to Human IgE, Heavy Chain Specific, 1 x 2 mL
9251 Antisera to Human (IgG, A, M, D, E) Pentavalent, Heavy Chain Specific, 1 x 2 mL
9257 Antisera to Human Lambda, LIght Chain Specific, 1 x 2 mL
9262 Antisera to Human Kappa, Light Chain Specific, 1 x 2 mL

Helena's antisera are produced in goats or sheep.


  • Titan Gel IEP Stain
Cat. No. Item
3049 TITAN GEL IEP Stain, 1 x 10 g

The stain is a Coomassie Brilliant Blue Stain in powder form designed especially for use with the TITAN GEL IEPlate Kit (Cat. No. 3047). Stain is dissolved in 1 L mixture of water, ethanol, and glacial acetic acid.

Remember:  IEP Sponge Wicks - Long (Cat. No. 9015), Dialamatic 1-10 μL Microdispenser (Cat. No. 6210), Electra B1 Buffer (cat. No. 5016),  Normal Human Serum Control (Cat. No. 9010),  Albumin Marker (Cat. No. 9011).


IEP Normal Human Serum Control and Albumin Marker

  • Normal Human Serum Control
Cat. No. Item
9010 Normal Human Serum Control, 1 x 2 mL
9011 Albumin Marker, 1 x 2 mL


The control is a stabilized liquid prepared from pooled normal human serum for use as a normal control serum for immunoelectrophoresis procedures. Albumin Marker is bromphenol blue which binds to albumin. The marker is added to the Normal Human Serum Control so the control can be used as a migration marker in immunoelectrophoresis procedures.

Remember:  IEP Sponge Wicks - Long (Cat. No. 9015),  Electra B1 Buffer (Cat. No. 5016).


4. TITAN GEL High Resolution Proteins

Titan gel high resolution protein gel

The major advantage of high resolution electrophoresis over conventional protein electrophoresis is that it gives more information about the patient's condition. Fifteen or so proteins can be visualized by use of high resolution, high sensitivity agarose gel electrophoresis of serum. It is the method of choice for detecting monoclonal immunoglobulins in serum and urine. The superior resolution and sensitivity of the procedure separates and detects proteins with similar electrophoretic mobilities and those present in low concentrations that may be invisible on other methods.


TITAN GEL High Resolution Protein Kit

  • Titan gel high resolution protein kit

Cat. No. Item Description
3040 TITAN GEL Hi Res Protein Kit 10 x 7 samples/gel


Kits include: 10 Gels, HR Buffer, HR Stain, Blotters, Wicks, Templates.

This method is excellent for detecting monoclonal immunoglobulins in serum and urine and oligoclonal bands in CSF. The procedure requires less than one hour for results and eliminates the need for expensive cumbersome cooling systems. This kit is used when sufficient CSF sample is available for concentration to 80 fold. Seven samples may be run on each gel.


High Resolution Protein Marker

  • High Resolution Protein Marker

Cat. No. Item Description
5141 High Resolution Protein Marker 1 x 0.1 mL

A qualitative marker for use with the TITAN GEL, REP and SPIFE High Resolution Protein Systems. The marker exhibits multiple bands and simulates oligoclonal banding in CSF and serum. It provides assurance that sufficient resolution has been achieved and is a visual reference for differentiating oligoclonal bands. The marker can be stained with Hi Res Protein Stain or Silver Stain. The marker is a stabilized liquid prepared from human serum.

Also for use with REP and SPIFE Hi Res assays.

Remember: TITAN GEL Chamber Cooling Device (Cat. No. 3039), Amido Black Protein stain (an alternate to Coomassie Brilliant Blue Stain (Cat. No. 3048).


5. TITAN GEL Alkaline Phosphatase Isoenzyme

Titan gel alkaline phosphatase isoenzyme gel


The alkaline phosphatase (ALP) isoenzymes found in human serum originate from several sources with the greatest activity occurring in the bone, liver, intestine, and placenta. Because of wide distribution of alkaline phosphatase in tissue, limited information can be obtained from a total ALP assay. Fortunately, the tissue sources of elevated ALP in serum can be determined by identifying the isoenzyme.

The isoenzymes of alkaline phosphatase are unique in that some organs have only one major isoenzyme rather than multiple isoenzyme forms. The isoenzymes of ALP differ in their physicochemical and electrophoretic properties, and it is by taking advantage of these differences that individual isoenzymes can be identified. In addition to the liver, bone, intestinal and placental isoenzymes, macrohepatic, Regan, PA, Nagao, and renal isoenzymes have also been identified in serum.

The resolution, accuracy and convenience of the TITAN GEL Alkaline Phosphatase High Resolution procedure make it better than heat denaturation, isoelectric focusing and wheat germ lectin techniques for separating ALP isoenzymes.  

TITAN GEL Alkaline Phosphatase Kit

  • Titan Gel Hi Res Alkaline Phosphatase kit
Cat. No. Item Description
3058 TITAN GEL Hi Res Alk Phos 10 x 8 samples/gel
  Isoenzyme Kit

Kits include: 10 Gels, ALP Buffer, ALP Separation Enhancer, ALP Reagent, ALP Diluent, Blotter, Wicks, Templates.

The TITAN GEL high resolution agarose electrophoresis method for separation of alkaline phosphatase isoenzymes provides excellent resolution. Neuraminidase is used to enhance separation of bone and liver fractions. A unique additive to the gel matrix ensures separation of the macrohepatic fraction from bone and liver. The excellent sensitivity of the system allows detection and separation of ALP isoenzymes even in samples with low activity. Up to eight samples may be applied per gel.


Alkaline Phosphatase Isoenzyme Control

  • alkaline Phosphatase Isoenzyme Control
Cat. No. Item Description
5104 Gel ALP Isoenzyme Control 1 x 2 mL

This control is for use as a qualitative and/or quantitative control to aid in the identification of alkaline phosphatase isoenzymes by agarose gel electrophoresis. It is prepared from pooled human serum and contains liver and bone isoenzyme bands. The control is a stabilized liquid.

Also for use with REP and SPIFE assays.